World Journal of Nephrology and Urology, ISSN 1927-1239 print, 1927-1247 online, Open Access
Article copyright, the authors; Journal compilation copyright, World J Nephrol Urol and Elmer Press Inc
Journal website http://www.wjnu.org

Original Article

Volume 5, Number 1, March 2016, pages 4-10

Voiding Dysfunction in Mice With Experimental Autoimmune Encephalomyelitis: A Multiple Sclerosis-Like Disease

Figures

Figure 1.
Figure 1. Curve of diameter/micturition volume. Calibration of micturition volume (µL) correlated with diameter of micturition, measured from wet circle on absorbent paper (mm) (mean ± standard deviation). Red line: regression curve.
Figure 2.
Figure 2. Mean urine volume per micturition. *P < 0.05
Figure 3.
Figure 3. Persistence of postvoid residual urine (PVR) in healthy and EAE mice. *P < 0.05.
Figure 4.
Figure 4. Postvoid residual urine (PVR) volume after 8 h (µL) in healthy mice and EAE mice. *P < 0.05.
Figure 5.
Figure 5. Correlation between clinical EAE stages and micturition diary variables. (A) Correlation between clinical EAE stages and number of 8-h micturitions. *P = 0.049. (B) Correlation between clinical EAE stages and 8-h urine output. *P = 0.029. (C) Correlation between clinical EAE stages and mean urine volume per micturition. **P = 0.009.
Figure 6.
Figure 6. Detrusor overactivity in healthy and EAE mice. ***P < 0.0001. DO: detrusor overactivity.

Table

Table 1. Results of Clinical and Urodynamic Investigations in Healthy and EAE Mice
 
Healthy miceEAE miceP
n: number in each group; %: percentage; PVR: postvoid residual urine ; NS: non-significant.
Mice (n)2036NS
Weight at time of clinical and cystometric investigation (g)20.58 ± 0.316.89 ± 0.240.0001
Micturition diary variables
  8-h micturitions, (n)2.1 ± 0.32.6 ± 0.2NS
  Total 8-h urine output (µL)623 ± 90601 ± 0.2NS
  Mean urine volume per micturition (µL)213 ± 17.5138.8 ± 200.018
  Persistence of PVR, % (n)30 (6/20)61 (22/36)0.049
  PVR volume (µL)197.5 ± 12260 ± 150.045
Cystometric variables
  Detrusor overactivity, % (n)10 (2)69 (25)< 0.0001
  Area under the curve1,632 ± 1762,475 ± 24NS
  Maximum bladder pressure (mm Hg/µL)45.7 ± 346.3 ± 3NS