Determination of Prooxidant and Antioxidant Balance, Clinical Parameters and Nutrient Intakes in Hemodialysis Patients

Abstract

Background: There is evidence that hemodialysis (HD) leads to oxidative stress. However, reports about balance between oxidant and antioxidant are contradictory. Thus, the aim of this study was to evaluate the prooxidant-antioxidant balance (PAB) in HD patients and its correlation with clinical parameters and food intakes.

Methods: This case-control study was performed in 35 HD patients and 35 healthy controls. Food intake was assessed by a 24-hour dietary recall questionnaire for at least 2 days. Routine biochemical parameters and C-reactive protein (CRP) were measured using the standard techniques. PAB was simultaneously measured by a new modified PAB assay using 3,3?,5,5?-tetramethylbenzidine benzidine (TMB).

Results: PAB in HD patients was significantly higher compared with control group (88.2 33 vs. 68.4 23.3, P < 0.01). A significant positive correlation was established between PAB values and serum levels of creatinine (r = 0.38, P < 0.001), blood urea nitrogen (BUN) (r = 0.42, P < 0.0001) and CRP (r = 0.34, P < 0.001). An inverse correlation was found between PAB values and intake of vitamins A (r = -0.28, P < 0.05) and C (r = -0.31, P < 0.01) in HD patients.

Conclusion: Since little data exits on the PAB assay in the HD patients, the high values of PAB indicated in our study need to be confirmed by further studies.




World J Nephrol Urol. 2015;4(2):207-212
doi: http://dx.doi.org/10.14740/wjnu194w